Journal: Neural Regeneration Research

Article Title: Melatonin lowers edema after spinal cord injury

doi: 10.4103/1673-5374.147954

Figure Lengend Snippet: Changes of aquaporin-4-immunoreactive cells at 24 and 72 hours after injury. (A) Immunohistochemical staining, optical microscope, × 200. (A) Arrows: Aquaporin-4-immunoreactive cells. (B) Changes in the number of aquaporin-4-immunoreactive cells. * P < 0.05, vs . sham surgery group; # P < 0.05, vs . SCI group. Data are expressed as the mean ± SD ( n = 5, one-way analysis of variance and the least significant difference test). SCI: Spinal cord injury.

Article Snippet: The membranes were blocked with 5% skim milk for 2 hours, and then incubated with polyclonal antibody rabbit anti-rat aquaporin-4 (1:400; Solarbio Biotechnology, Beijing, China), rabbit anti-rat GFAP (1:200; Solarbio Biotechnology) or β-actin (1:2,000; Solarbio Biotechnology).

Techniques: Immunohistochemical staining, Staining, Microscopy

Journal: Neural Regeneration Research

Article Title: Melatonin lowers edema after spinal cord injury

doi: 10.4103/1673-5374.147954

Figure Lengend Snippet: Western blot assay for AQP4 and GFAP protein levels at 12 (A), 24 (B), 48 (C) and 72 hours (D) after injury. The graph showed significant up-regulation of AQP4 and GFAP expression in the SCI group at 12, 24, 48 and 72 hours after SCI. Treatment of melatonin (100 mg/kg) could markedly decrease AQP4 and GFAP levels in the spinal cord at 24, 48 and 72 hours after injury. * P < 0.05, vs . sham surgery (sham) group; # P < 0.05, vs . SCI group. Data are expressed as the mean ± SD ( n = 5, one-way analysis of variance and the least significant difference test). SCI: Spinal cord injury; AQP4: aquaporin-4; GFAP: glial fibrillary acidic protein.

Article Snippet: The membranes were blocked with 5% skim milk for 2 hours, and then incubated with polyclonal antibody rabbit anti-rat aquaporin-4 (1:400; Solarbio Biotechnology, Beijing, China), rabbit anti-rat GFAP (1:200; Solarbio Biotechnology) or β-actin (1:2,000; Solarbio Biotechnology).

Techniques: Western Blot, Expressing

Journal: Molecular Biology of the Cell

Article Title: Galectin-3, a Novel Centrosome-associated Protein, Required for Epithelial Morphogenesis

doi: 10.1091/mbc.E09-03-0193

Figure Lengend Snippet: Defects in renal epithelial cell organization in galectin-3 null mutant mice. Confocal microscopy analysis of mouse kidney sections. Distribution of Na + /K + -ATPase (A), gp114 (B), aquaporin-2 (AQ-2; C), villin (D), and α-tubulin (E), in wild-type (wt) or galectin-3 null mutant ( gal3 −/−) mouse kidneys. Nuclei were detected by Hoechst 33342 staining. Renal tubules were delimited with a white line along basal membranes. The borders of a single cell are indicated with a dotted line in E. Scale bars, 10 μm.

Article Snippet: Rabbit anti-centrin-2 antibodies were from Sigma (St. Louis, MO) and from Santa Cruz Biotechnology (Santa Cruz, CA), rabbit anti-pericentrin from Covance (Berkeley, CA), rabbit anti-aquaporin-2 from United States Biological (Swampscott, MA), rabbit anti-Na + /K + -ATPase from Abcam (Cambridge, MA), monoclonal anti-α- and β-tubulin, and anti-acetylated-α-tubulin, or rabbit γ-tubulin were from Sigma and monoclonal anti-flotillin-1 from BD Biosciences (Rockville, MD).

Techniques: Mutagenesis, Confocal Microscopy, Staining